Real Methods

This type of protocol bridges the gap between what is given in a “Methods” section of a manuscript, and what researchers actually do. Primarily, these protocols show specific applications of established approaches. These protocols are linked to publications or contain sample data.

Preparing Conducting Polymers for Sensing Applications

Genotyping Aag-/- Mice from Ear/Tail

Genotyping Aag-/- Mice from Ear/Tail A protocol for genotyping Aag-/- mice from ear punches or tail clippings. The Aag gene codes for alkyladenine DNA glycosylase, an enzyme that initiates repair of a variety of DNA lesions via the base excision repair pathway.

Genotyping AagTg+/- Mice from Ear/Tail

Genotyping AagTg+/- Mice from Ear/Tail A protocol for genotyping AagTg+/- mice from ear punches or tail clippings. AagTg transgeneic animals have increased alkyladenine DNA glycosylase (Aag) activity via promoter-controlled expression of Aag cDNA.

Genotyping Mgmt-/- Mice from Ear/Tail

Genotyping Mgmt-/- Mice from Ear/Tail A protocol for genotyping Mgmt-/- mice from ear punches or tail clippings. The Mgmt gene codes for O6-alkylguanine DNA alkyltransferase, which converts mutagenic O6-methylguanine lesions in DNA to guanine by demethylation.

Staining for Ki67 in paraffin-embedded tissue sections

Staining for Ki67 in paraffin-embedded tissue sections This protocol describes how to immunofluorescence stain for Ki67, a marker of cellular proliferation, in paraffin-embedded tissue sections.

Making cell line samples for proteomic analysis

Making cell line samples for proteomic analysis This protocol describes how to make cell line lysates for downstream proteomic analysis by mass spectrometry. In this protocol, BaP is used as an example for treatment, but the process can be applied for other treatments.

Treating TK6 Cells with Lanthanide Chlorides (for use with CometChip protocol)

Treating TK6 Cells with Lanthanide Chlorides (for use with CometChip protocol) Description of how to prepare the cells and lanthanide solutions used for studying DNA damaging potential of these metals.

RaDR Mouse Necropsy and Imaging

RaDR Mouse Necropsy and Imaging This protocol describes how to collect tissues for imaging EGFP foci in RaDR mice. Includes tips for clean excision and removing autofluorescent substances.

Immunofluorescent staining on paraffin-embedded tissue sections

Immunofluorescent staining on paraffin-embedded tissue sections This protocol describes the steps necessary to fluorescently detect proteins in paraffin-embedded tissue sections. After depariffinizing and rehydrating the tissues, antigens are exposed through incubation with boiling antigen retrieval buffer. Subsequently, sections are blocked with bovine serum albumin and probed with primary antibody overnight. After a series of washes, […]