NextGen Protocols

Share more detailed information than a methods section.

Integrate short smartphone videos.

Accelerate science by sharing new methods.

Propel new technologies.

Why Use NextGen Protocols

Why Use NextGen Protocols?

  • Appropriate for all levels of expertise, from fundamental laboratory techniques to cutting edge methods for emerging technologies
  • Unique multimedia format including written and video elements make it easy to master new techniques
  • Utility and reproducibility are paramount
  • Accessible from your desktop, tablet, smartphone, or printed page
  • Safety guidelines are included
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Protocol Categories

Fundamental Techniques
Primarily for use in teaching and as a vehicle for students to demonstrate the quality of their work, this type of protocol usually covers established laboratory techniques.

Novel Technologies
This type of protocol bridges the gap between what is given in a “Methods” section of a manuscript, and what researchers actually do. Primarily, these protocols show specific applications of established approaches. These protocols are linked to publications or contain sample data.

Real Methods
These protocols provide detail about innovative approaches such that other researchers will be able to successfully adopt the approach. These protocols are linked to publications that demonstrate the efficacy of the approach.

Why publish a NextGen Protocol?

Why Publish a NextGen Protocol?

  • Free and open to all
  • Fast from concept to publication
  • Easy step-by-step instructions for creating and publishing your protocol using just your computer and a smartphone; no special equipment required.
  • Codify standard protocols; originality is welcome, but not required.
  • Accommodates wet and dry lab protocols
  • Credits you for your work
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What else do we offer?

What else do we offer?

  • Access to standard operating procedures
  • Safety guidelines
  • Key points regarding responsible research conduct and your responsibilities
  • Advice on notebooks
  • Advice on intellectual property protection
  • Opportunities in support of our global research community
  • Lots of tips on how to become a great scientist! :)
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Featured Protocols

Staining for γH2AX in paraffin-embedded tissue sections

This protocol describes how to stain sections of formalin-fixed paraffin-embedded tissue for phosphorylated Ser139 on histone variant H2A.X (γH2AX). A primary antibody is used to identify and bind to γH2AX, and a secondary antibody conjugated to a fluorescent probe identifies and binds the primary antibody for imaging by fluorescence.

 
Preparing a Chemiresistive Sensor Based on Functionalized Carbon Nanotubes

This protocol describes how to covalently functionalize single-wall carbon nanotubes with iodonium salt and fabricate a CNT-based chemiresistive sensor for the detection of carbon monoxide.

 
Making cell line samples for proteomic analysis

This protocol describes how to make cell line lysates for downstream proteomic analysis by mass spectrometry. In this protocol, BaP is used as an example for treatment, but the process can be applied for other treatments.

 
RaDR Mouse Necropsy and Imaging

This protocol describes how to collect tissues for imaging EGFP foci in RaDR mice. Includes tips for clean excision and removing autofluorescent substances.

 
Single Cell Microarray for High Throughput Detection of DNA Damage

Motivation
• DNA damage is associated with an increased risk of cancer, aging and disease
• Few methods are available for high throughput analysis of DNA damage
• This protocol is based upon the traditional comet assay wherein DNA migrates more
readily through a matrix when damaged
• 96 samples can be processed in parallel
• Throughput and sensitivity are increased compared to the traditional assay

 
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