Fundamental Techniques
Primarily for use in teaching and as a vehicle for students to demonstrate the quality of their work, this type of protocol usually covers established laboratory techniques.
Novel Technologies
This type of protocol bridges the gap between what is given in a “Methods” section of a manuscript, and what researchers actually do. Primarily, these protocols show specific applications of established approaches. These protocols are linked to publications or contain sample data.
Real Methods
These protocols provide detail about innovative approaches such that other researchers will be able to successfully adopt the approach. These protocols are linked to publications that demonstrate the efficacy of the approach.
This protocol describes how to stain sections of formalin-fixed paraffin-embedded tissue for phosphorylated Ser139 on histone variant H2A.X (γH2AX). A primary antibody is used to identify and bind to γH2AX, and a secondary antibody conjugated to a fluorescent probe identifies and binds the primary antibody for imaging by fluorescence.