NextGen Protocols

Share more detailed information than a methods section.

Integrate short smartphone videos.

Accelerate science by sharing new methods.

Propel new technologies.

Why Use NextGen Protocols

Why Use NextGen Protocols?

  • Appropriate for all levels of expertise, from fundamental laboratory techniques to cutting edge methods for emerging technologies
  • Unique multimedia format including written and video elements make it easy to master new techniques
  • Utility and reproducibility are paramount
  • Accessible from your desktop, tablet, smartphone, or printed page
  • Safety guidelines are included

Protocol Categories

Fundamental Techniques
Primarily for use in teaching and as a vehicle for students to demonstrate the quality of their work, this type of protocol usually covers established laboratory techniques.

Novel Technologies
This type of protocol bridges the gap between what is given in a “Methods” section of a manuscript, and what researchers actually do. Primarily, these protocols show specific applications of established approaches. These protocols are linked to publications or contain sample data.

Real Methods
These protocols provide detail about innovative approaches such that other researchers will be able to successfully adopt the approach. These protocols are linked to publications that demonstrate the efficacy of the approach.

Why publish a NextGen Protocol?

Why Publish a NextGen Protocol?

  • Free and open to all
  • Fast from concept to publication
  • Easy step-by-step instructions for creating and publishing your protocol using just your computer and a smartphone; no special equipment required.
  • Codify standard protocols; originality is welcome, but not required.
  • Accommodates wet and dry lab protocols
  • Credits you for your work
What else do we offer?

What else do we offer?

  • Access to standard operating procedures
  • Safety guidelines
  • Key points regarding responsible research conduct and your responsibilities
  • Advice on notebooks
  • Advice on intellectual property protection
  • Opportunities in support of our global research community
  • Lots of tips on how to become a great scientist! :)

Featured Protocols

Disaggregation of intact colon crypts

This protocol describes how to disaggregate whole colon tissue into a suspension of colonic crypts. This protocol was adapted from Samuel et al., Am J Physiol Cell Physiol 296: C296-C305, 2009. The sample image shows a suspension of crypts, with intact crypts outlined in red.

Genotyping Aag-/- Mice from Ear/Tail

A protocol for genotyping Aag-/- mice from ear punches or tail clippings. The Aag gene codes for alkyladenine DNA glycosylase, an enzyme that initiates repair of a variety of DNA lesions via the base excision repair pathway.

Genotyping AagTg+/- Mice from Ear/Tail

A protocol for genotyping AagTg+/- mice from ear punches or tail clippings. AagTg transgeneic animals have increased alkyladenine DNA glycosylase (Aag) activity via promoter-controlled expression of Aag cDNA.

Genotyping Mgmt-/- Mice from Ear/Tail

A protocol for genotyping Mgmt-/- mice from ear punches or tail clippings. The Mgmt gene codes for O6-alkylguanine DNA alkyltransferase, which converts mutagenic O6-methylguanine lesions in DNA to guanine by demethylation.