Genotyping Aag-/- Mice from Ear/Tail

LEAD AUTHOR Joshua J. Corrigan, Technical Associate, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA USA PROTOCOL TYPE:
Protocol Document
SYNOPSIS: A protocol for genotyping Aag-/- mice from ear punches or tail clippings. The Aag gene codes for alkyladenine DNA glycosylase, an enzyme that initiates repair of a variety of DNA lesions via the base excision repair pathway.
PUBLICATIONS: Allocca M, Corrigan JJ, Fake KR, Calvo JA, Samson LD. PARP inhibitors protect against sex- and AAG-dependent alkylation-induced neural degeneration. Oncotarget. 2017;8(40):68707-68720. Published 2017 Aug 3. doi:10.18632/oncotarget.19844

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