Novel Technologies

These protocols provide detail about innovative approaches such that other researchers will be able to successfully adopt the approach. These protocols are linked to publications that demonstrate the efficacy of the approach.

Preparing a Chemiresistive Sensor Based on Functionalized Carbon Nanotubes

Preparing a Chemiresistive Sensor Based on Functionalized Carbon Nanotubes

Preparing a Chemiresistive Sensor Based on Functionalized Carbon Nanotubes This protocol describes how to covalently functionalize single-wall carbon nanotubes with iodonium salt and fabricate a CNT-based chemiresistive sensor for the detection of carbon monoxide.  

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Making cell line samples for proteomic analysis

Making cell line samples for proteomic analysis

Making cell line samples for proteomic analysis This protocol describes how to make cell line lysates for downstream proteomic analysis by mass spectrometry. In this protocol, BaP is used as an example for treatment, but the process can be applied for other treatments.  

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RaDR Mouse Necropsy and Imaging

RaDR Mouse Necropsy and Imaging

RaDR Mouse Necropsy and Imaging This protocol describes how to collect tissues for imaging EGFP foci in RaDR mice. Includes tips for clean excision and removing autofluorescent substances.  

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PCR to genotype RaDR mouse from ear punch

PCR to genotype RaDR mouse from ear punch

PCR to genotype RaDR mouse from ear punch These protocols describe the process to genotype for the RaDR transgene in mouse ear punches. RaDR mice were developed in the Engelward lab at MIT, and they allow detection of sequence rearrangement mutations arising from aberrant homologous recombination in situ. Please see the paper below for more […]

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Single Cell Microarray for High Throughput Detection of DNA Damage

Single Cell Microarray for High Throughput Detection of DNA Damage

Single Cell Microarray for High Throughput Detection of DNA Damage Motivation • DNA damage is associated with an increased risk of cancer, aging and disease • Few methods are available for high throughput analysis of DNA damage • This protocol is based upon the traditional comet assay wherein DNA migrates more readily through a matrix […]

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