This type of protocol bridges the gap between what is given in a “Methods” section of a manuscript, and what researchers actually do. Primarily, these protocols show specific applications of established approaches. These protocols are linked to publications or contain sample data.
RNA-seq sample preparation for MiSeq sequencing (Illumina) using SPIA amplification method RNA extraction from tissue or low yield samples and preparation of RNA samples for RNA-sequencing (RNA-seq) using NuGEN’s SPIA (Single Primer Isothermal Amplification) amplification technology.
Preparing Mouse Organs for Flow Cytometry This protocol describes how to disaggregate fresh tissues into single cell suspensions for analysis by flow cytometry. The protocol was developed for analyzing the proportion of EGFP+ cells in RaDR mouse tissues, but it can be adapted to any application requiring a suspension of single cells.