Making cell line samples for proteomic analysis

LEAD AUTHOR

Ishwar Kohale
Graduate Student,
Department of Biological Engineering,
Massachusetts Institute of Technology,
Cambridge MA

ikohale@mit.edu

OTHER AUTHORS:

Forest White
Professor,
Department of Biological Engineering,
Koch Institute for Integrative Cancer Research,
Massachusetts Institute of Technology,
Cambridge MA

PROTOCOL TYPE:
Protocol Document
SYNOPSIS:

This protocol describes how to make cell line lysates for downstream proteomic analysis by mass spectrometry. In this protocol, BaP is used as an example for treatment, but the process can be applied for other treatments.