Genotyping AagTg+/- Mice from Ear/Tail

LEAD AUTHOR Joshua J. Corrigan, Technical Associate, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA USA

Protocol Document
SYNOPSIS: A protocol for genotyping AagTg+/- mice from ear punches or tail clippings. AagTg transgeneic animals have increased alkyladenine DNA glycosylase (Aag) activity via promoter-controlled expression of Aag cDNA.
PUBLICATIONS: Calvo JA, Moroski-Erkul CA, Lake A, Eichinger LW, Shah D, Jhun I, et al. (2013) Aag DNA Glycosylase Promotes Alkylation-Induced Tissue Damage Mediated by Parp1. PLoS Genet 9(4): e1003413. Allocca M, Corrigan JJ, Fake KR, Calvo JA, Samson LD. PARP inhibitors protect against sex- and AAG-dependent alkylation-induced neural degeneration. Oncotarget. 2017;8(40):68707-68720. Published 2017 Aug 3. doi:10.18632/oncotarget.19844

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