Genotyping AagTg+/- Mice from Ear/Tail

 
LEAD AUTHOR

Joshua J. Corrigan, Technical Associate, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA USA


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corrigan@mit.edu

PROTOCOL TYPE: Real Methods
Protocol Document
SYNOPSIS:

A protocol for genotyping AagTg+/- mice from ear punches or tail clippings. AagTg transgeneic animals have increased alkyladenine DNA glycosylase (Aag) activity via promoter-controlled expression of Aag cDNA.


 
PUBLICATIONS:

Calvo JA, Moroski-Erkul CA, Lake A, Eichinger LW, Shah D, Jhun I, et al. (2013) Aag DNA Glycosylase Promotes Alkylation-Induced Tissue Damage Mediated by Parp1. PLoS Genet 9(4): e1003413. https://doi.org/10.1371/journal.pgen.1003413

Allocca M, Corrigan JJ, Fake KR, Calvo JA, Samson LD. PARP inhibitors protect against sex- and AAG-dependent alkylation-induced neural degeneration. Oncotarget. 2017;8(40):68707-68720. Published 2017 Aug 3. doi:10.18632/oncotarget.19844


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