Genotyping Mgmt-/- Mice from Ear/Tail

 
LEAD AUTHOR

Joshua J. Corrigan, Technical Associate, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA USA


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corrigan@mit.edu

PROTOCOL TYPE:
Protocol Document
SYNOPSIS:

A protocol for genotyping Mgmt-/- mice from ear punches or tail clippings. The Mgmt gene codes for O6-alkylguanine DNA alkyltransferase, which converts mutagenic O6-methylguanine lesions in DNA to guanine by demethylation.


 

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