RaDR Mouse Necropsy and Imaging

LEAD AUTHOR Jennifer Kay, PhD. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA USA jekay@mit.edu

OTHER AUTHORS: Michelle Sukup Jackson, PhD. MIT, Cambridge, MA Orsolya Kiraly, PhD. MIT, Cambridge, MA Professor Bevin Engelward, MIT, Cambridge, MA
Protocol Document
Click here for example data file.
SYNOPSIS: This protocol describes how to collect tissues for imaging EGFP foci in RaDR mice. Includes tips for clean excision and removing autofluorescent substances.
PUBLICATIONS: Sukup-Jackson MR, Kiraly O, Kay JE, Na L, Rowland EA, et al. (2014) Rosa26-GFP Direct Repeat (RaDR-GFP) Mice Reveal Tissue- and Age-Dependence of Homologous Recombination in Mammals In Vivo. PLoS Genet 10(6): e1004299. doi: 10.1371/journal.pgen.1004299

Tags: , , , ,

Post a Reply

Your email address will not be published. Required fields are marked *