RaDR Mouse Necropsy and Imaging
LEAD AUTHOR
OTHER AUTHORS:
PROTOCOL TYPE:
Protocol Document
Click here for example data file.
SYNOPSIS:
Jennifer Kay, PhD. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA USA
jekay@mit.eduOTHER AUTHORS:
Michelle Sukup Jackson, PhD. MIT, Cambridge, MA
Orsolya Kiraly, PhD. MIT, Cambridge, MA
Professor Bevin Engelward, MIT, Cambridge, MA
PROTOCOL TYPE:
Protocol Document
Click here for example data file.
SYNOPSIS:
This protocol describes how to collect tissues for imaging EGFP foci in RaDR mice. Includes tips for clean excision and removing autofluorescent substances.
Sukup-Jackson MR, Kiraly O, Kay JE, Na L, Rowland EA, et al. (2014) Rosa26-GFP Direct Repeat (RaDR-GFP) Mice Reveal Tissue- and Age-Dependence of Homologous Recombination in Mammals In Vivo. PLoS Genet 10(6): e1004299. doi: 10.1371/journal.pgen.1004299