Fabricating the 96-Well CometChip
Fabricating the 96-Well CometChip This protocol details how to fabricate the 96-well CometChip.
DNA damage
CometChip Data Analysis – Running the Guicometanalyzer in Debug mode
CometChip Data Analysis – Running the Guicometanalyzer in Debug mode Any time CometChip is performed on a new cell type, it is important to confirm that you are using appropriate parameters for analyzing your data set. This can be done by choosing a representative well image and analyzing it in a special “debug” mode in […]
CometChip Data Analysis Using the Guicometanalyzer
CometChip Data Analysis Using the Guicometanalyzer The CometChip is a high-throughput version of the comet assay and is used to measure DNA damage. This protocol takes you through running the Guicometanalyzer code for analyzing CometChip images. The Guicometanalyzer code is freely available and can be found at the provided link: The Guicometanalyzer is available at […]
Treating TK6 Cells with Lanthanide Chlorides (for use with CometChip protocol)
Treating TK6 Cells with Lanthanide Chlorides (for use with CometChip protocol) Description of how to prepare the cells and lanthanide solutions used for studying DNA damaging potential of these metals.
Single Cell Microarray for High Throughput Detection of DNA Damage
Single Cell Microarray for High Throughput Detection of DNA Damage Motivation • DNA damage is associated with an increased risk of cancer, aging and disease • Few methods are available for high throughput analysis of DNA damage • This protocol is based upon the traditional comet assay wherein DNA migrates more readily through a matrix […]