Staining

Staining for γH2AX in paraffin-embedded tissue sections

Staining for γH2AX in paraffin-embedded tissue sections

Staining for γH2AX in paraffin-embedded tissue sections This protocol describes how to stain sections of formalin-fixed paraffin-embedded tissue for phosphorylated Ser139 on histone variant H2A.X (γH2AX). A primary antibody is used to identify and bind to γH2AX, and a secondary antibody conjugated to a fluorescent probe identifies and binds the primary antibody for imaging by […]

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Staining for Ki67 in paraffin-embedded tissue sections

Staining for Ki67 in paraffin-embedded tissue sections

Staining for Ki67 in paraffin-embedded tissue sections This protocol describes how to immunofluorescence stain for Ki67, a marker of cellular proliferation, in paraffin-embedded tissue sections.  

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Immunofluorescent staining on paraffin-embedded tissue sections

Immunofluorescent staining on paraffin-embedded tissue sections

Immunofluorescent staining on paraffin-embedded tissue sections This protocol describes the steps necessary to fluorescently detect proteins in paraffin-embedded tissue sections. After depariffinizing and rehydrating the tissues, antigens are exposed through incubation with boiling antigen retrieval buffer. Subsequently, sections are blocked with bovine serum albumin and probed with primary antibody overnight. After a series of washes, […]

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