Staining for γH2AX in paraffin-embedded tissue sections

LEAD AUTHOR Jennifer Kay, PhD Massachusetts Institute of Technology, Cambridge MA

OTHER AUTHORS: Joshua Corrigan, Massachusetts Institute of Technology
Protocol Document
Click here for example data file.
SYNOPSIS: This protocol describes how to stain sections of formalin-fixed paraffin-embedded tissue for phosphorylated Ser139 on histone variant H2A.X (γH2AX). A primary antibody is used to identify and bind to γH2AX, and a secondary antibody conjugated to a fluorescent probe identifies and binds the primary antibody for imaging by fluorescence.
SAFETY PROCEDURES: This protocol involves use of xylenes. Wear appropriate PPE and work with xylenes in a chemical hood. Dispose of xylenes through appropriate hazardous waste stream.

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