Staining for γH2AX in paraffin-embedded tissue sections
Staining for γH2AX in paraffin-embedded tissue sections This protocol describes how to stain sections of formalin-fixed paraffin-embedded tissue for phosphorylated Ser139 on histone variant H2A.X (γH2AX). A primary antibody is used to identify and bind to γH2AX, and a secondary antibody conjugated to a fluorescent probe identifies and binds the primary antibody for imaging by […]
