Single Cell Microarray for High Throughput Detection of DNA Damage


Bevin P. Engelward (MIT-Cambridge)
Principal Investigator
Biological Engineering
Cambridge, MA USA


Jing Ge, Danielle Chow
Jessica Fessler

PROTOCOL TYPE: Novel Technologies
Protocol Document

• DNA damage is associated with an increased risk of cancer, aging and disease
• Few methods are available for high throughput analysis of DNA damage
• This protocol is based upon the traditional comet assay wherein DNA migrates more
readily through a matrix when damaged
• 96 samples can be processed in parallel
• Throughput and sensitivity are increased compared to the traditional assay


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Cycle, 12:907-915(2013).
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using microwell arrays, Proc. Natl. Acad. Sci. USA,107:10008-10013 (2010).


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