Genotyping AagTg+/- Mice from Ear/Tail A protocol for genotyping AagTg+/- mice from ear punches or tail clippings. AagTg transgeneic animals have increased alkyladenine DNA glycosylase (Aag) activity via promoter-controlled expression of Aag cDNA.
Staining for Ki67 in paraffin-embedded tissue sections This protocol describes how to immunofluorescence stain for Ki67, a marker of cellular proliferation, in paraffin-embedded tissue sections.
Treating TK6 Cells with Lanthanide Chlorides (for use with CometChip protocol) Description of how to prepare the cells and lanthanide solutions used for studying DNA damaging potential of these metals.
Immunofluorescent Staining for γH2AX in Cultured Cells
RNA-seq sample preparation for MiSeq sequencing (Illumina) using SPIA amplification method RNA extraction from tissue or low yield samples and preparation of RNA samples for RNA-sequencing (RNA-seq) using NuGEN’s SPIA (Single Primer Isothermal Amplification) amplification technology.
Preparing Mouse Organs for Flow Cytometry This protocol describes how to disaggregate fresh tissues into single cell suspensions for analysis by flow cytometry. The protocol was developed for analyzing the proportion of EGFP+ cells in RaDR mouse tissues, but it can be adapted to any application requiring a suspension of single cells.