Preparing Mouse Organs for Flow Cytometry
LEAD AUTHOR Jennifer Kay, Graduate student, MIT Department of Biological Engineering, Cambridge, MA, USA
jekay@mit.edu
OTHER AUTHORS: Dr. Michelle Sukup-Jackson, former graduate student Dr. Orsolya Kiraly, former post-doc Dr. Bevin Engelward, Professor All from MIT Department of Biological Engineering, Cambridge, MA
PROTOCOL TYPE: Real Methods
Protocol Document
Click here for example data file.
SYNOPSIS: This protocol describes how to disaggregate fresh tissues into single cell suspensions for analysis by flow cytometry. The protocol was developed for analyzing the proportion of EGFP+ cells in RaDR mouse tissues, but it can be adapted to any application requiring a suspension of single cells.
ABSTRACT: This protocol is a description of the Real Methods used to disaggregate tissues into single cell suspensions. In order to perform flow cytometric analysis on single cells from mice, it is necessary to mechanically mince the tissue and chemically digest connective components while leaving cells alive and intact. This protocol provides information to create cell suspensions from pancreas, liver, breast, intestine, spleen, brain, kidney, thymus, and lung tissues.
OTHER AUTHORS: Dr. Michelle Sukup-Jackson, former graduate student Dr. Orsolya Kiraly, former post-doc Dr. Bevin Engelward, Professor All from MIT Department of Biological Engineering, Cambridge, MA
PROTOCOL TYPE: Real Methods
Protocol Document
Click here for example data file.
SYNOPSIS: This protocol describes how to disaggregate fresh tissues into single cell suspensions for analysis by flow cytometry. The protocol was developed for analyzing the proportion of EGFP+ cells in RaDR mouse tissues, but it can be adapted to any application requiring a suspension of single cells.
ABSTRACT: This protocol is a description of the Real Methods used to disaggregate tissues into single cell suspensions. In order to perform flow cytometric analysis on single cells from mice, it is necessary to mechanically mince the tissue and chemically digest connective components while leaving cells alive and intact. This protocol provides information to create cell suspensions from pancreas, liver, breast, intestine, spleen, brain, kidney, thymus, and lung tissues.