LEAD AUTHOR Jennifer Kay, Doctoral candidate. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA USA
jekay@mit.edu
OTHER AUTHORS:
Takafumi Kimoto, Teijin Pharma Ltd, Tokyo Japan
PROTOCOL TYPE:
Protocol Document
Click here for example data file.
SYNOPSIS:
This protocol describes how to disaggregate whole colon tissue into a suspension of colonic crypts. This protocol was adapted from Samuel et al., Am J Physiol Cell Physiol 296: C296-C305, 2009. The sample image shows a suspension of crypts, with intact crypts outlined in red.
PUBLICATIONS:
Samuel et al., Am J Physiol Cell Physiol 296: C296-C305, 2009
Sukup-Jackson MR, Kiraly O, Kay JE, Na L, Rowland EA, Winther KE, et al. (2014) Rosa26-GFP Direct Repeat (RaDR-GFP) Mice Reveal Tissue- and Age-Dependence of Homologous Recombination in Mammals In Vivo. PLoS Genet 10(6): e1004299. doi:10.1371/journal.pgen.1004299
Tags: colon, crypt, crypt suspension, disaggregate