Disaggregation of intact colon crypts

 
LEAD AUTHOR

Jennifer Kay, Doctoral candidate. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA USA


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jekay@mit.edu

OTHER AUTHORS:

Takafumi Kimoto, Teijin Pharma Ltd, Tokyo Japan


PROTOCOL TYPE:
Protocol Document
Click here for example data file.
SYNOPSIS:

This protocol describes how to disaggregate whole colon tissue into a suspension of colonic crypts. This protocol was adapted from Samuel et al., Am J Physiol Cell Physiol 296: C296-C305, 2009. The sample image shows a suspension of crypts, with intact crypts outlined in red.


 
PUBLICATIONS:

Samuel et al., Am J Physiol Cell Physiol 296: C296-C305, 2009

Sukup-Jackson MR, Kiraly O, Kay JE, Na L, Rowland EA, Winther KE, et al. (2014) Rosa26-GFP Direct Repeat (RaDR-GFP) Mice Reveal Tissue- and Age-Dependence of Homologous Recombination in Mammals In Vivo. PLoS Genet 10(6): e1004299. doi:10.1371/journal.pgen.1004299


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