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Staining for γH2AX in paraffin-embedded tissue sections

Staining for γH2AX in paraffin-embedded tissue sections

Staining for γH2AX in paraffin-embedded tissue sections This protocol describes how to stain sections of formalin-fixed paraffin-embedded tissue for phosphorylated Ser139 on histone variant H2A.X (γH2AX). A primary antibody is used to identify and bind to γH2AX, and a secondary antibody conjugated to a fluorescent probe identifies and binds the primary antibody for imaging by […]

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Preparing a Chemiresistive Sensor Based on Functionalized Carbon Nanotubes

Preparing a Chemiresistive Sensor Based on Functionalized Carbon Nanotubes

Preparing a Chemiresistive Sensor Based on Functionalized Carbon Nanotubes This protocol describes how to covalently functionalize single-wall carbon nanotubes with iodonium salt and fabricate a CNT-based chemiresistive sensor for the detection of carbon monoxide.  

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Making cell line samples for proteomic analysis

Making cell line samples for proteomic analysis

Making cell line samples for proteomic analysis This protocol describes how to make cell line lysates for downstream proteomic analysis by mass spectrometry. In this protocol, BaP is used as an example for treatment, but the process can be applied for other treatments.  

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RaDR Mouse Necropsy and Imaging

RaDR Mouse Necropsy and Imaging

RaDR Mouse Necropsy and Imaging This protocol describes how to collect tissues for imaging EGFP foci in RaDR mice. Includes tips for clean excision and removing autofluorescent substances.  

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Single Cell Microarray for High Throughput Detection of DNA Damage

Single Cell Microarray for High Throughput Detection of DNA Damage

Single Cell Microarray for High Throughput Detection of DNA Damage Motivation • DNA damage is associated with an increased risk of cancer, aging and disease • Few methods are available for high throughput analysis of DNA damage • This protocol is based upon the traditional comet assay wherein DNA migrates more readily through a matrix […]

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RNA-seq sample preparation for MiSeq sequencing (Illumina) using SPIA amplification method

RNA-seq sample preparation for MiSeq sequencing (Illumina) using SPIA amplification method

RNA-seq sample preparation for MiSeq sequencing (Illumina) using SPIA amplification method RNA extraction from tissue or low yield samples and preparation of RNA samples for RNA-sequencing (RNA-seq) using NuGEN’s SPIA (Single Primer Isothermal Amplification) amplification technology.  

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Why NextGen Protocols?

Why NextGen Protocols?

Paper gets the key information across…. but time is wasted when text alone doesn’t convey the subtleties of how to handle samples.

In-house video protocols are helpful, but rarely have enough detail to be followed successfully.

Platforms for professional, annotated videography are available, but researchers can’t always access these expensive tools.

Mechanisms for sharing screen-capture execution of dry-lab experiments are lacking.

NextGen Protocols meet these challenges with a low barrier to entry approach that allows researchers to create multimedia protocols that integrate video and written elements using readily available tools such as cell phone video cameras and word processing.

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