Staining for γH2AX in paraffin-embedded tissue sections
LEAD AUTHOR Jennifer Kay, PhD
Massachusetts Institute of Technology, Cambridge MA
jekay@mit.edu
OTHER AUTHORS: Joshua Corrigan, Massachusetts Institute of Technology
PROTOCOL TYPE:
Protocol Document
Click here for example data file.
SYNOPSIS: This protocol describes how to stain sections of formalin-fixed paraffin-embedded tissue for phosphorylated Ser139 on histone variant H2A.X (γH2AX). A primary antibody is used to identify and bind to γH2AX, and a secondary antibody conjugated to a fluorescent probe identifies and binds the primary antibody for imaging by fluorescence.
OTHER AUTHORS: Joshua Corrigan, Massachusetts Institute of Technology
PROTOCOL TYPE:
Protocol Document
Click here for example data file.
SYNOPSIS: This protocol describes how to stain sections of formalin-fixed paraffin-embedded tissue for phosphorylated Ser139 on histone variant H2A.X (γH2AX). A primary antibody is used to identify and bind to γH2AX, and a secondary antibody conjugated to a fluorescent probe identifies and binds the primary antibody for imaging by fluorescence.