Staining for γH2AX in paraffin-embedded tissue sections

 
LEAD AUTHOR

Jennifer Kay, PhD
Massachusetts Institute of Technology, Cambridge MA


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jekay@mit.edu

OTHER AUTHORS:

Joshua Corrigan, Massachusetts Institute of Technology


PROTOCOL TYPE:
Protocol Document
Click here for example data file.
SYNOPSIS:

This protocol describes how to stain sections of formalin-fixed paraffin-embedded tissue for phosphorylated Ser139 on histone variant H2A.X (γH2AX). A primary antibody is used to identify and bind to γH2AX, and a secondary antibody conjugated to a fluorescent probe identifies and binds the primary antibody for imaging by fluorescence.


 
SAFETY PROCEDURES:

This protocol involves use of xylenes. Wear appropriate PPE and work with xylenes in a chemical hood. Dispose of xylenes through appropriate hazardous waste stream.


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