Fabricating the 96-Well CometChip
Fabricating the 96-Well CometChip This protocol details how to fabricate the 96-well CometChip.
Novel Technologies
These protocols provide detail about innovative approaches such that other researchers will be able to successfully adopt the approach. These protocols are linked to publications that demonstrate the efficacy of the approach.
CometChip Data Analysis – Running the Guicometanalyzer in Debug mode
CometChip Data Analysis – Running the Guicometanalyzer in Debug mode Any time CometChip is performed on a new cell type, it is important to confirm that you are using appropriate parameters for analyzing your data set. This can be done by choosing a representative well image and analyzing it in a special “debug” mode in […]
CometChip Data Analysis Using the Guicometanalyzer
CometChip Data Analysis Using the Guicometanalyzer The CometChip is a high-throughput version of the comet assay and is used to measure DNA damage. This protocol takes you through running the Guicometanalyzer code for analyzing CometChip images. The Guicometanalyzer code is freely available and can be found at the provided link: The Guicometanalyzer is available at […]
Preparing a Chemiresistive Sensor Based on Functionalized Carbon Nanotubes
Preparing a Chemiresistive Sensor Based on Functionalized Carbon Nanotubes This protocol describes how to covalently functionalize single-wall carbon nanotubes with iodonium salt and fabricate a CNT-based chemiresistive sensor for the detection of carbon monoxide.
Making cell line samples for proteomic analysis
Making cell line samples for proteomic analysis This protocol describes how to make cell line lysates for downstream proteomic analysis by mass spectrometry. In this protocol, BaP is used as an example for treatment, but the process can be applied for other treatments.
RaDR Mouse Necropsy and Imaging
RaDR Mouse Necropsy and Imaging This protocol describes how to collect tissues for imaging EGFP foci in RaDR mice. Includes tips for clean excision and removing autofluorescent substances.
PCR to genotype RaDR mouse from ear punch
PCR to genotype RaDR mouse from ear punch These protocols describe the process to genotype for the RaDR transgene in mouse ear punches. RaDR mice were developed in the Engelward lab at MIT, and they allow detection of sequence rearrangement mutations arising from aberrant homologous recombination in situ. Please see the paper below for more […]
Single Cell Microarray for High Throughput Detection of DNA Damage
Single Cell Microarray for High Throughput Detection of DNA Damage Motivation • DNA damage is associated with an increased risk of cancer, aging and disease • Few methods are available for high throughput analysis of DNA damage • This protocol is based upon the traditional comet assay wherein DNA migrates more readily through a matrix […]