Genotyping Aag-/- Mice from Ear/Tail A protocol for genotyping Aag-/- mice from ear punches or tail clippings. The Aag gene codes for alkyladenine DNA glycosylase, an enzyme that initiates repair of a variety of DNA lesions via the base excision repair pathway.
Genotyping AagTg+/- Mice from Ear/Tail A protocol for genotyping AagTg+/- mice from ear punches or tail clippings. AagTg transgeneic animals have increased alkyladenine DNA glycosylase (Aag) activity via promoter-controlled expression of Aag cDNA.
Genotyping Mgmt-/- Mice from Ear/Tail A protocol for genotyping Mgmt-/- mice from ear punches or tail clippings. The Mgmt gene codes for O6-alkylguanine DNA alkyltransferase, which converts mutagenic O6-methylguanine lesions in DNA to guanine by demethylation.
PCR to genotype RaDR mouse from ear punch These protocols describe the process to genotype for the RaDR transgene in mouse ear punches. RaDR mice were developed in the Engelward lab at MIT, and they allow detection of sequence rearrangement mutations arising from aberrant homologous recombination in situ. Please see the paper below for more […]