disaggregate

Disaggregation of intact colon crypts

Disaggregation of intact colon crypts

Disaggregation of intact colon crypts This protocol describes how to disaggregate whole colon tissue into a suspension of colonic crypts. This protocol was adapted from Samuel et al., Am J Physiol Cell Physiol 296: C296-C305, 2009. The sample image shows a suspension of crypts, with intact crypts outlined in red.  

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