Western Blot with BioRad Protean 3 Cell
LEAD AUTHOR
OTHER AUTHORS:
PROTOCOL TYPE:
Protocol Document
Click here for example data file.
SYNOPSIS:
Jennifer Kay, Doctoral candidate. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA USA
jekay@mit.eduOTHER AUTHORS:
Marcus Parrish, Doctoral Candidate, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA USA
PROTOCOL TYPE:
Protocol Document
Click here for example data file.
SYNOPSIS:
This protocol describes how to perform a Western blot using the BioRad Protean 3 Cell system, one of many available Western blot assemblies. The basic steps of this protocol can be adapted to any Western blot assembly, but we recommend reading the instructions for your system first.
This protocol involves use of methanol. Please review safety guidelines for methanol use, and be sure to discard methanol-containing solutions in appropriate waste containers.