Disaggregation of intact colon crypts
LEAD AUTHOR Jennifer Kay, Doctoral candidate. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA USA
jekay@mit.edu
OTHER AUTHORS: Takafumi Kimoto, Teijin Pharma Ltd, Tokyo Japan
PROTOCOL TYPE:
Protocol Document
Click here for example data file.
SYNOPSIS: This protocol describes how to disaggregate whole colon tissue into a suspension of colonic crypts. This protocol was adapted from Samuel et al., Am J Physiol Cell Physiol 296: C296-C305, 2009. The sample image shows a suspension of crypts, with intact crypts outlined in red.
OTHER AUTHORS: Takafumi Kimoto, Teijin Pharma Ltd, Tokyo Japan
PROTOCOL TYPE:
Protocol Document
Click here for example data file.
SYNOPSIS: This protocol describes how to disaggregate whole colon tissue into a suspension of colonic crypts. This protocol was adapted from Samuel et al., Am J Physiol Cell Physiol 296: C296-C305, 2009. The sample image shows a suspension of crypts, with intact crypts outlined in red.