NextGen Protocols

Share more detailed information than a methods section.

Integrate short smartphone videos.

Accelerate science by sharing new methods.

Propel new technologies.

Why Use NextGen Protocols

Why Use NextGen Protocols?

  • Appropriate for all levels of expertise, from fundamental laboratory techniques to cutting edge methods for emerging technologies
  • Unique multimedia format including written and video elements make it easy to master new techniques
  • Utility and reproducibility are paramount
  • Accessible from your desktop, tablet, smartphone, or printed page
  • Safety guidelines are included
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Protocol Categories

Fundamental Techniques
Primarily for use in teaching and as a vehicle for students to demonstrate the quality of their work, this type of protocol usually covers established laboratory techniques.

Novel Technologies
This type of protocol bridges the gap between what is given in a “Methods” section of a manuscript, and what researchers actually do. Primarily, these protocols show specific applications of established approaches. These protocols are linked to publications or contain sample data.

Real Methods
These protocols provide detail about innovative approaches such that other researchers will be able to successfully adopt the approach. These protocols are linked to publications that demonstrate the efficacy of the approach.

Why publish a NextGen Protocol?

Why Publish a NextGen Protocol?

  • Free and open to all
  • Fast from concept to publication
  • Easy step-by-step instructions for creating and publishing your protocol using just your computer and a smartphone; no special equipment required.
  • Codify standard protocols; originality is welcome, but not required.
  • Accommodates wet and dry lab protocols
  • Credits you for your work
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What else do we offer?

What else do we offer?

  • Access to standard operating procedures
  • Safety guidelines
  • Key points regarding responsible research conduct and your responsibilities
  • Advice on notebooks
  • Advice on intellectual property protection
  • Opportunities in support of our global research community
  • Lots of tips on how to become a great scientist! :)
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Featured Protocols

Disaggregation of intact colon crypts

This protocol describes how to disaggregate whole colon tissue into a suspension of colonic crypts. This protocol was adapted from Samuel et al., Am J Physiol Cell Physiol 296: C296-C305, 2009. The sample image shows a suspension of crypts, with intact crypts outlined in red.

 
 
Immunofluorescent staining on paraffin-embedded tissue sections

This protocol describes the steps necessary to fluorescently detect proteins in paraffin-embedded tissue sections. After depariffinizing and rehydrating the tissues, antigens are exposed through incubation with boiling antigen retrieval buffer. Subsequently, sections are blocked with bovine serum albumin and probed with primary antibody overnight. After a series of washes, the sections are incubated with secondary antibody and detected with a fluorescent microscope.

 
PCR to genotype RaDR mouse from ear punch

This is the protocol to genotype for the RaDR transgene in mouse ear punches. RaDR mice were developed in the Engelward lab at MIT, and they allow detection of sequence rearrangement mutations arising from aberrant homologous recombination in situ. Please see the paper below for more information on RaDR.

 
Preparing Mouse Organs for Flow Cytometry

This protocol describes how to disaggregate fresh tissues into single cell suspensions for analysis by flow cytometry. The protocol was developed for analyzing the proportion of EGFP+ cells in RaDR mouse tissues, but it can be adapted to any application requiring a suspension of single cells.

 
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